Single Nucleotide Polymorphism  at  rs7903146 of Transcription Factor 7-like 2 gene Among Subjects with  Type 2 Diabetes Mellitus in Myanmar

Sagawah Phu; Aye Thida; Kyu Kyu Maung; Tet Tun Chit

doi:10.15605/jafes.037.S2

Authors

Sagawah Phu Department of Biochemistry, University of Medicine Taunggyi https://orcid.org/0000-0002-1985-1564
Aye Thida University of Medicine 2, Yangon, Myanmar https://orcid.org/0000-0002-6390-8193
Kyu Kyu Maung University of Medicine 1, Yangon, Myanmar
Tet Tun Chit East Yangon General Hospital, Yangon, Myanmar

DOI:

https://doi.org/10.15605/jafes.037.S2

Keywords:

TCF7L2, SNP, Myanmar

Abstract

Objectives. To investigate the association between the single nucleotide polymorphism (SNP) rs7903146 in the transcription factor 7-like 2 (TCF7L2) gene and type 2 diabetes mellitus (T2DM) and to examine the impact of this variant on pancreatic beta-cell function in the Myanmar population.

Methodology. A case-control study was undertaken in 100 subjects with T2DM and 113 controls. The SNP rs7903146 was genotyped using the allele-specific polymerase chain reaction method. Plasma glucose and serum insulin levels were determined using the enzymatic colorimetric method and ELISA respectively. Beta-cell function was calculated by the HOMA-β formula.

Results. The frequencies of carrier genotypes (CT and TT) were higher in subjects with T2DM than in controls. The minor T alleles of rs7903146 were found to statistically increase type 2 diabetes risk than the C allele with an allelic odds ratio of 2.07 (95% CI 1.39-3.09, p=0.0004). The mean HOMA-β level of the group with non-carrier genotype (CC) was significantly higher than that of the groups with carrier genotypes (CT and TT) in subjects with T2DM and controls with a p-value of 0.0003 and less than 0.0001, respectively.

Conclusion. The rs7903146 variant of the TCF7L2 gene was found to be associated with T2DM and low β-cell function among Myanmar subjects.

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Author Biographies

Aye Thida, University of Medicine 2, Yangon, Myanmar

Professor and Head, Department of Medical Education

Kyu Kyu Maung, University of Medicine 1, Yangon, Myanmar

Professor and Head, Department of Biochemistry

Tet Tun Chit, East Yangon General Hospital, Yangon, Myanmar

Senior Consultant Physician, Department of Medicine

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