Angiotensin I-Converting Enzyme Gene Polymorphism and Diabetic Nephropathy in Filipino Type 2 Diabetes Mellitus Patients

Authors

  • Elizabeth Paz-Pacheco Section of Endocrinology, Diabetes and Metabolism; Department of Medicine; University of the Philippines – Philippine General Hospital; Manila, Philippines;
  • Eva Maria Cutiongco-de la Paz Institute of Human Genetics, National Institutes of Health, University of the Philippines Manila, Philippines
  • Gabriel V. Jasul, Jr. Section of Endocrinology, Diabetes and Metabolism; Department of Medicine; University of the Philippines – Philippine General Hospital; Manila, Philippines;
  • Ma. Cecille S. Añonuevo-Cruz Section of Endocrinology, Diabetes and Metabolism; Department of Medicine; University of the Philippines – Philippine General Hospital; Manila, Philippines;
  • Rhoda L. Montemayor Section of Endocrinology, Diabetes and Metabolism; Department of Medicine; University of the Philippines – Philippine General Hospital; Manila, Philippines;

Abstract

Objective. Previous studies have demonstrated the role of genetic susceptibility in the pathogenesis of diabetic nephropathy. The study aimed to determine the frequencies of angiotensin I-converting enzyme (ACE) gene insertion/deletion (I/D) polymorphism in a pilot population of Filipino type 2 diabetic patients and normal controls.

 

Methods. An analysis of the ACE gene polymorphism was performed in 42 diabetic patients with and without nephropathy, and 24 normal controls. The analysis was done using polymerase chain reaction, restriction enzyme digestion, and gel electrophoresis techniques to determine the polymorphism (II, DD or ID). Independent T-tests and chi-square tests were used to compare clinical characteristics, and logistic regression analysis was done to determine odds ratio for development of nephropathy.

 

Results. The ID polymorphism of the ACE gene was more frequent (52.4%) in patients with diabetic nephropathy (n=21). In those without nephropathy (n=21), II was more common (61.9%). ID was the more frequent genotype in the normal controls (n=24) (58.3%). The odds of developing diabetic nephropathy were increased by 4.8 times in those with ID polymorphism, and 2.9 times in those with DD.

 

Conclusion. The D allele was more common in patients with diabetic nephropathy, similar to the observation in South Indian patients. Since the study involved only a small pilot group, studies on a larger population is needed to establish the hypothesized role of the D allele in susceptibility to diabetic nephropathy in Filipinos.

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References

American Diabetes Association. Standards of medical care in diabetes 2006. Diabetes Care 2006;29(Suppl1):S4-42.

Renal Disease Control Project (REDCOP). Philippine Renal Disease Registry 2005. Unpublished data.

American Diabetes Association. Nephropathy in diabetes. Diabetes Care 2004;27(Suppl1):S79-83.

Seaquist ER, Goetz FC, Rich S et al. Familial clustering of diabetic kidney disease: Evidence of genetic susceptibility to diabetic nephropathy. N Engl J Med 1989;320:1161-5.

Borch-Johnsen K, Nørgaard K, Hommel E et al. Is diabetic nephropathy an inherited complication? Kidney Int 1992;41:719-2.

DeGroot LJ, Jameson JL, eds. Endocrinology. 5th edition. Philadelphia: Elsevier Saunders, 2006.

Tarnow L, Gluud C, Parving H. Diabetic nephropathy and the insertion/deletion polymorphism of the angiotensin-converting enzyme gene. Nephrol Dial Transplant 1998;13:1125-30.

Hsieh M, Lin S, Hsieh T et al. Increased frequency of angiotensin-converting enzyme DD genotype in patients with type 2 diabetes in Taiwan. Nephrol Dial Transplant 2000;15:1008-13.

Rigat B, Hubert C, Alhenc-Gelas F et al. An insertion/deletion polymorphism in the angiotensin I- converting enzyme gene accounting for half the variance of serum enzyme levels. J Clin Invest 1990;86:1343-6.

Brenner BM, Meyer TW, Hostetter TH. Dietary protein intake and the progressive nature of kidney disease: The role of hemodynamically mediated glomerular injury in the pathogenesis of progressive glomerular sclerosis in aging, renal ablation, and intrinsic renal disease. N Engl J Med 1982;307:652-60.

Solini A, Vestra MD, Saller A et al. The angiotensin-converting enzyme DD genotype is associated with glomerulopathy lesions in type 2 diabetes. Diabetes 2002;51:251-5.

Viswanathan V, Zhu Y, Bala K et al. Association between ACE gene polymorphism and diabetic nephropathy in South Indian patients. J Pancreas 2001;2(2):83-7.

Ng DPK, Tai BC, Koh D et al. Angiotensin-I converting enzyme insertion/deletion polymorphism and its association with diabetic nephropathy: A meta-analysis of studies reported between 1994 and 2004 and comprising 14,727 subjects. Diabetologia 2005;48:1008-16.

American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2004;27(Suppl1):S5-10.

Paz-Pacheco E, Cutiongco-de la Paz EMC, Halili-Manabat C et al. Mitochondrial DNA (T/C) 16189 polymorphism, variants and heteroplasmy among Filipinos with type 2 diabetes mellitus. Acta Medica Philippina 2008;42(1):17-21.

Mogensen CE, Viberti GC, Peheim E et al. Multicenter evaluation of the Micral-Test II test strip, an immunologic rapid test for the detection of microalbuminuria. Diabetes Care 1997;20(11):1642-6.

Cavalli-Sforza LL, Piazza A, Menozzi P, Mountain J. Reconstruction of human evolution: Bringing together genetic, archaeological, and linguistic data. Proc Natl Acad Sci USA 1988;85:6002-6.

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Published

2014-05-22

How to Cite

Paz-Pacheco, E., Cutiongco-de la Paz, E. M., Jasul, Jr., G. V., S. Añonuevo-Cruz, M. C., & Montemayor, R. L. (2014). Angiotensin I-Converting Enzyme Gene Polymorphism and Diabetic Nephropathy in Filipino Type 2 Diabetes Mellitus Patients. Journal of the ASEAN Federation of Endocrine Societies, 27(1), 87. Retrieved from https://asean-endocrinejournal.org/index.php/JAFES/article/view/19

Issue

Vol. 27 No. 1 (2012): May Issue

Section

Original Articles

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